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cd44  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cd44
    Cd44, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44/CD44+Antibody/pmc13052523-51-17-26
    Average 96 stars, based on 789 article reviews
    cd44 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Aronia Berry Extract Inhibits Cancer Stemness and Overcomes 5-Fluorouracil Resistance by Targeting TLR3/NF-κB Signaling in Colorectal Cancer
    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in Tris-buffered saline (BIO-RAD) containing 0.1% Tween-20 (Sigma-Aldrich) for 1 h at room temperature. .. Primary antibodies, including anti-CD44 (1:1000, 5023S; Cell Signaling Technology [CST], Danvers, MA, USA), anti-Nanog (1:1000, 9505; CST), anti-Oct4 (1:1000, 9532S; CST), anti-TLR3 (1:1000, PA5-20183; Thermo Fisher Scientific), anti-P65 (1:1000, 3034; CST), anti-GAPDH (1:2000, 5174T; CST), anti-β-actin (1:5000, 58169S; CST), and anti-Lamin A/C (1:1000, 10298-1-AP; Proteintech, Rosemont, IL, USA) were incubated with the membranes at 4 ◦C overnight. .. After three washes with TBST, the membrane was incubated with the appropriate anti-rabbit (1:2000, 7074; CST) or anti-mouse (1:2000, 7076; CST) secondary antibodies for 1 h at room temperature.

    Article Title: Aronia Berry Extract Inhibits Cancer Stemness and Overcomes 5-Fluorouracil Resistance by Targeting TLR3/NF-κB Signaling in Colorectal Cancer
    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in Tris-buffered saline (BIO-RAD) containing 0.1% Tween-20 (Sigma-Aldrich) for 1 h at room temperature. .. Primary antibodies, including anti-CD44 (1:1000, 5023S; Cell Signaling Technology [CST], Danvers, MA, USA), anti-Nanog (1:1000, 9505; CST), anti-Oct4 (1:1000, 9532S; CST), anti-TLR3 (1:1000, PA5-20183; Thermo Fisher Scientific), anti-P65 (1:1000, 3034; CST), anti-GAPDH (1:2000, 5174T; CST), anti-β-actin (1:5000, 58169S; CST), and anti-Lamin A/C (1:1000, 10298-1-AP; Proteintech, Rosemont, IL, USA) were incubated with the membranes at 4 °C overnight. .. After three washes with TBST, the membrane was incubated with the appropriate anti-rabbit (1:2000, 7074; CST) or anti-mouse (1:2000, 7076; CST) secondary antibodies for 1 h at room temperature.

    Staining:

    Article Title: Caloric restriction attenuates oxidative and inflammatory markers of aging, while compromising germinative epithelium homeostasis in the rat aged testis.
    Article Snippet: 1 Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro 88100, Italy 2 Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende 87036, Italy 3 Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende 87036, Italy 4 Department of Mathematics and Computer Science, University of Calabria, Rende (Cosenza) 87036, Italy 5 Pathology Unit, Annunziata Hospital, Cosenza 87100, Italy 6 Department of Surgery, Division of Urology, Annunziata Hospital, Cosenza 87100, Italy 7 Department of Health Sciences, Magna Graecia University of Catanzaro, Catanzaro 88100, Italy 8 Endocrine and Metabolic Diseases Unit, Annunziata Hospital, Cosenza 87100, Italy Abstract Purpose Aging is accompanied by a chronic low-grade systemic inflammatory state known as “inflammaging”, driven by the accumulation of pro-inflammatory and immunosenescent cells, oxidative stress, and dysregulation of apoptosis and autophagy.. Experimental evidence suggests that caloric restriction (CR) can slow down inflammaging, although its impact on spermatogenesis remains controversial.. This study aimed to investigate the effects of CR on age-related testicular inflammation and any effects on germ cells.

    Immunostaining:

    Article Title: Caloric restriction attenuates oxidative and inflammatory markers of aging, while compromising germinative epithelium homeostasis in the rat aged testis.
    Article Snippet: 1 Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro 88100, Italy 2 Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende 87036, Italy 3 Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende 87036, Italy 4 Department of Mathematics and Computer Science, University of Calabria, Rende (Cosenza) 87036, Italy 5 Pathology Unit, Annunziata Hospital, Cosenza 87100, Italy 6 Department of Surgery, Division of Urology, Annunziata Hospital, Cosenza 87100, Italy 7 Department of Health Sciences, Magna Graecia University of Catanzaro, Catanzaro 88100, Italy 8 Endocrine and Metabolic Diseases Unit, Annunziata Hospital, Cosenza 87100, Italy Abstract Purpose Aging is accompanied by a chronic low-grade systemic inflammatory state known as “inflammaging”, driven by the accumulation of pro-inflammatory and immunosenescent cells, oxidative stress, and dysregulation of apoptosis and autophagy.. Experimental evidence suggests that caloric restriction (CR) can slow down inflammaging, although its impact on spermatogenesis remains controversial.. This study aimed to investigate the effects of CR on age-related testicular inflammation and any effects on germ cells.



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    Miltenyi Biotec cd44 antibody
    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and <t>CD44</t> cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
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    Cell Signaling Technology Inc cd44
    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and <t>CD44</t> cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
    Cd44, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Journal: STAR Protocols

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

    doi: 10.1016/j.xpro.2026.104519

    Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Article Snippet: CD44 Antibody, anti-mouse, PE-Vio 770 clone IM7.8.1; 1:10 dilution , Miltenyi Biotec , Cat# 130-102-377 RRID: AB_2658187.

    Techniques: Cell Culture, Dot Blot, Luminex